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Mouse N-ras genes: organization of the functional locus and of a truncated cDNA-like pseudogene
H Y Chang1, I Guerrero, R Lake
1Department of Biochemistry, New York University School of Medicine, New York 10016.
Abstract:
The N-ras gene was first identified in both humans and mice as a mutationally activated oncogene found in tumors. The nucleotide sequence and intron/exon structure of the coding region of the mouse gene have been determined previously. We have now determined the sequence and intron/exon structure of the 5' and 3' untranslated regions of mouse N-ras. Like its human homolog, the 3' untranslated region of the gene is encoded by two exons, and the 5' region is encoded by one. In addition, we have isolated and sequenced a second mouse gene homologous to N-ras. This locus, which we have named N-ras-2ps, resides at a chromosomal site distinct from N-ras and appears to be a truncated cDNA-like pseudogene.
Insights
Researchers characterized the mouse N-ras gene, detailing its 5' and 3' untranslated regions. A second, distinct N-ras pseudogene (N-ras-2ps) was also identified and sequenced.
Area of Science:
- Genetics
- Molecular Biology
- Oncology
Background:
- The N-ras gene, a known oncogene implicated in tumor formation, has been previously studied in humans and mice.
- The coding region and intron/exon structure of the mouse N-ras gene were already established.
Purpose of the Study:
- To determine the sequence and intron/exon structure of the 5' and 3' untranslated regions of the mouse N-ras gene.
- To identify and characterize any additional homologous N-ras loci in the mouse genome.
Main Methods:
- DNA sequencing to determine the nucleotide sequence of the 5' and 3' untranslated regions of mouse N-ras.
- Comparative sequence analysis to identify homologous genes.
- Chromosomal mapping to determine the location of identified loci.
Main Results:
- The complete intron/exon structure of the mouse N-ras gene, including its 5' and 3' untranslated regions, was elucidated.
- The 5' untranslated region is encoded by one exon, and the 3' untranslated region by two exons, mirroring its human counterpart.
- A second homologous mouse gene, N-ras-2ps, was discovered and sequenced; it is located on a different chromosome and appears to be a pseudogene.
Conclusions:
- The comprehensive characterization of the mouse N-ras gene provides a more complete understanding of its genetic structure.
- The identification of the N-ras-2ps pseudogene suggests potential complexities in ras gene family regulation or evolution.
- Further research into N-ras-2ps may offer insights into gene duplication and pseudogene formation.