Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Structural features of ribonucleotide reductase.

I Nikas1, J McLauchlan, A J Davison

  • 1MRC Virology Unit, Institute of Virology, Glasgow, Scotland, U.K.

Proteins
|December 1, 1986
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Human cytomegalovirus multiple-strain infections and viral population diversity in haematopoietic stem cell transplant recipients analysed by high-throughput sequencing.

Medical microbiology and immunology·2021
Same author

Integrated morphologic and molecular analysis of Trichomonas vaginalis, Mycoplasma hominis, and human papillomavirus using cytologic smear preparations.

Parasitology research·2018
Same author

Genetic diversity of equine herpesvirus 1 isolated from neurological, abortigenic and respiratory disease outbreaks.

Transboundary and emerging diseases·2018
Same author

Cohort Profile: The Hepatitis C Virus (HCV) Research UK Clinical Database and Biobank.

International journal of epidemiology·2017
Same author

Anti-envelope antibody responses in individuals at high risk of hepatitis C virus who resist infection.

Journal of viral hepatitis·2016
Same author

Bioinformatics tools for analysing viral genomic data.

Revue scientifique et technique (International Office of Epizootics)·2016
Same journal

BioMatics 1.0: A Wasserstein Distance Approach for Next-Generation Multiple Sequence Alignment.

Proteins·2026
Same journal

Engineered HSP90-MP65 Bivalent Fusion Antigen: A Novel Vaccine Candidate Against Invasive Candidiasis.

Proteins·2026
Same journal

Physics-Based Energy Functions for Computational Protein Design.

Proteins·2026
Same journal

Impact of Stabilizing Osmolytes on the Conformational Dynamics of Human and Rat Islet Amyloid Polypeptides.

Proteins·2026
Same journal

Stabilization of Bone Morphogenetic Protein-2 at Physiological pH: Contrasting Roles of CHAPS and Arginine in Aggregation Inhibition.

Proteins·2026
Same journal

Structural Insights Into the Function of Leishmania major Adenylosuccinate Lyase.

Proteins·2026
See all related articles

Herpes simplex virus type 1 (HSV-1) ribonucleotide reductase (RR1 and RR2) DNA sequences were determined. Comparisons with varicella-zoster virus (VZV) revealed conserved domains important for enzyme activity and unique N-terminal regions in HSV-1.

Area of Science:

  • Virology
  • Molecular Biology
  • Biochemistry

Background:

  • Herpes simplex virus type 1 (HSV-1) encodes a ribonucleotide reductase (RR) essential for DNA synthesis.
  • The HSV-1 RR is composed of two subunits: RR1 (136,000 mol. wt.) and RR2 (38,000 mol. wt.).

Purpose of the Study:

  • To determine the complete DNA sequence of HSV-1 RR1.
  • To identify and characterize homologous genes in varicella-zoster virus (VZV).
  • To analyze amino acid sequence homologies and predict structural features of viral RR subunits.

Main Methods:

  • DNA sequencing of HSV-1 RR1.
  • Identification of open reading frames in VZV DNA with homology to HSV RR genes.
  • Amino acid sequence comparisons and computational structure predictions.

Related Experiment Videos

Main Results:

  • The complete DNA sequence for HSV-1 RR1 was determined.
  • Two homologous open reading frames were identified in VZV, predicting VZV RR1 (87,000 mol. wt.) and RR2 (35,000 mol. wt.).
  • HSV-1 RR1 possesses a unique N-terminal domain absent in other RR1s (except HSV-2), with distinct structural predictions and poor inter-herpesvirus conservation.
  • Conserved amino acid blocks across viral RR1 and RR2 subunits suggest functional importance, with a proposed substrate binding site in RR1 centered on conserved glycine residues.

Conclusions:

  • The study elucidates the genetic basis of HSV-1 and VZV ribonucleotide reductases.
  • Structural predictions highlight conserved regions critical for enzyme function and unique viral-specific domains.
  • The promoters for HSV and Epstein-Barr virus (EBV) RR2 transcripts likely evolved independently.