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Quantification of Cell Turnover in the Bovine Leukemia Virus Model
Alix de Brogniez1, Pierre-Yves Barez1, Alexandre Carpentier1
1Molecular Biology (GxABT) and Molecular and Cellular Epigenetics (GIGA), University of Liege, Avenue de l'Hôpital 1 B34, 4000, Gembloux and Liege, Belgium.
Methods in Molecular Biology (Clifton, N.J.)
|March 31, 2017
Abstract:
In a perspective of a comparative virology approach, characterization of the bovine leukemia virus (BLV) model may be helpful to better understand infection by the related human T-lymphotropic virus type 1 (HTLV-1). In this paper, we first provide detailed protocols to inoculate cloned BLV proviruses into sheep or cattle. We also describe methods to quantify apoptosis ex vivo and cell turnover in vivo.