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Updated: Aug 8, 2026

Preparation and Use of HIV-1 Infected Primary CD4+ T-Cells as Target Cells in Natural Killer Cell Cytotoxic Assays
Published on: March 14, 2011
Analysis of NK Cell Function and Receptor Expression During HTLV-1 and HTLV-2 Infection
Federica Bozzano1, Francesco Marras1, Andrea De Maria2
1Molecular Immunology, University of Genova, Viale Benedetto XV 9, 16132, Genoa, Italy.
Abstract:
Cytofluorimetric analysis is a typical method in immunology to evaluate phenotype and function of Natural Killer (NK) cells derived from HTLV-1/2 infected patients and healthy donors. Here, we described protocols to NK cells phenotypical and cytotoxicity assay, performed by flow cytometry on fresh and immune-magnetically or flow cytometry sorted NK cells. A new developed protocol able to evaluate IFNγ production has been included.
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