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Isolation of Epithelial Cells from Human Dental Follicle

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Published on: November 5, 2021

Functional Tooth Regeneration.

Masamitsu Oshima^1,2, Miho Ogawa^2,3, Takashi Tsuji^4,5

¹Department of Oral Rehabilitation and Regenerative Medicine, Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, Okayama, 700-8525, Japan.

Methods in Molecular Biology (Clifton, N.J.)

|April 1, 2017

Summary

This summary is machine-generated.

Researchers developed a novel organ germ method to precisely replicate organogenesis. This technique successfully generated a fully functional bioengineered tooth, offering new avenues for regenerative medicine and developmental biology research.

Keywords:

Bioengineered tooth Cell manipulation Organ germ method Organ replacement regenerative therapy Tooth regeneration Transplantation

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Area of Science:

Developmental Biology
Bioengineering
Regenerative Medicine

Background:

Three-dimensional organogenesis relies on spatiotemporal regulation of cellular behaviors and signaling molecules.
Epithelial-mesenchymal interactions are crucial for organ development and morphogenesis.
Current bioengineering aims to replicate organogenesis for creating functional bioengineered organs from stem cells.

Purpose of the Study:

To develop a novel three-dimensional cell manipulation method for precise organogenesis replication.
To generate a structurally correct and fully functional bioengineered tooth in vivo.
To establish protocols for tooth germ reconstitution and functional analysis of tooth development.

Main Methods:

Development of the 'organ germ method' for three-dimensional cell manipulation.
Reconstitution of tooth germs using the organ germ method.
In vitro and in vivo functional analysis of tooth development.

Main Results:

Successful generation of a structurally correct and fully functional bioengineered tooth in vivo.
Demonstration of the organ germ method's capability to replicate organogenesis.
Establishment of protocols for tooth germ reconstitution and analysis.

Conclusions:

The organ germ method enables precise replication of organogenesis.
This method facilitates the creation of functional bioengineered organs, specifically a tooth.
The technique is valuable for analyzing gene and protein functions during organogenesis.