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Related Concept Videos

RNA Stability01:53

RNA Stability

Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...
RNA Stability01:53

RNA Stability

Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...
mRNA Stability and Gene Expression02:51

mRNA Stability and Gene Expression

The structure and stability of mRNA molecules regulates gene expression, as mRNAs are a key step in the pathway from gene to protein. In eukaryotes, the half-life of mRNA varies from a few minutes up to several days. mRNA stability is essential in growth and development. The absence of the proteins regulating its stability, such as tristetraprolin in mice, can cause systemic issues, including bone marrow overgrowth, inflammation, and autoimmunity.
Cis-acting Elements involved in mRNA stability
mRNA Stability and Gene Expression02:51

mRNA Stability and Gene Expression

The structure and stability of mRNA molecules regulates gene expression, as mRNAs are a key step in the pathway from gene to protein. In eukaryotes, the half-life of mRNA varies from a few minutes up to several days. mRNA stability is essential in growth and development. The absence of the proteins regulating its stability, such as tristetraprolin in mice, can cause systemic issues, including bone marrow overgrowth, inflammation, and autoimmunity.
Cis-acting Elements involved in mRNA stability
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
Regulation of Expression at Multiple Steps01:23

Regulation of Expression at Multiple Steps

The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the addition of a...

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Updated: Jul 1, 2026

Analysis of Global RNA Synthesis at the Single Cell Level following Hypoxia
14:53

Analysis of Global RNA Synthesis at the Single Cell Level following Hypoxia

Published on: May 13, 2014

Hypoxia and Hypoglycemia synergistically regulate mRNA stability.

Kristen R Carraway1, Ellen M Johnson1, Travis C Kauffmann2

  • 1a Biochemistry and Molecular Biology, Brody School of Medicine , East Carolina University , Greenville , NC , USA.

RNA Biology
|April 1, 2017
PubMed
Summary

Researchers identified novel ischemia-related messenger RNAs (mRNAs) stabilized by combined oxygen and glucose deprivation. This posttranscriptional regulation involves KHSRP release, crucial for tissue survival during ischemic events.

Keywords:
HypoxiaKHSRPhypoglycemiamRNA stability

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Area of Science:

  • Molecular Biology
  • Cellular Physiology
  • Biochemistry

Background:

  • Ischemic events, characterized by reduced blood flow, impair oxygen and glucose delivery to tissues.
  • While transcriptional responses to ischemia are well-studied, posttranscriptional regulation remains less understood.
  • Investigating mRNA stability offers insights into cellular adaptation during oxygen and glucose deprivation.

Purpose of the Study:

  • To explore the posttranscriptional regulation of mRNA stability under conditions of oxygen and glucose deprivation.
  • To identify specific messenger RNAs (mRNAs) involved in this ischemia-related response.

Main Methods:

  • Experimental induction of hypoxia (low oxygen) and hypoglycemia (low glucose) in cellular models.
  • Measurement of mRNA half-life for key ischemia-related genes.
  • Analysis of RNA-binding protein interactions, specifically KHSRP, with target mRNAs.

Main Results:

  • Identified novel ischemia-related mRNAs, including VEGF, MYC, MDM2, and CYR61, synergistically stabilized by combined hypoxia and hypoglycemia.
  • Demonstrated that simultaneous low oxygen (1%) and low glucose (≤ 1 g/L) are required for synergistic mRNA stabilization.
  • Observed release of these stabilized mRNAs from the RNA-binding protein KHSRP under hypoxia/hypoglycemia.

Conclusions:

  • Combined oxygen and glucose deprivation triggers a unique posttranscriptional regulatory mechanism.
  • Stabilization of specific mRNAs like VEGF, MYC, MDM2, and CYR61 by hypoxia/hypoglycemia is mediated by KHSRP release.
  • This regulation of mRNA stability is a critical adaptive response to ischemic conditions.