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A Device for Performing Cell Migration/Wound Healing in a 96-Well Plate
Po Ying Poon1, Patrick Ying Kit Yue1, Ricky Ngok Shun Wong2
1Department of Biology, Hong Kong Baptist University.
Journal of Visualized Experiments : Jove
|April 1, 2017
Summary
This study introduces an 8-channel mechanical wounder for cell migration assays. The device ensures reproducible cell wounding, enhancing studies of cell migration, angiogenesis, and tumor metastasis.
Area of Science:
- Cell Biology
- Biotechnology
Background:
- Cell migration assays are crucial for studying cell movement, angiogenesis, and tumor metastasis.
- Traditional methods face challenges in reproducibility and contamination.
Purpose of the Study:
- To develop an improved mechanical wounder for cell migration assays.
- To address limitations of existing cell wounding techniques.
Main Methods:
- Utilized an 8-channel mechanical wounder with adjustable pins and guiding bars.
- Employed disposable plastic pipette tips for wounding in 96-well plates.
- Sterilization achieved via autoclaving or disinfectants.
Main Results:
- The device creates sharp and reproducible wounds.
- Ensures consistent wounding position across wells.
- Minimizes cross-contamination and offers user-friendly handling.
Conclusions:
- The novel cell wounder provides a user-friendly and reproducible tool for cell migration assays.
- Facilitates standard cell migration studies using 96-well plates.