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A guide to light-sheet fluorescence microscopy for multiscale imaging.

Rory M Power1,2,3, Jan Huisken1,2,3

  • 1Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany.

Nature Methods
|April 1, 2017
PubMed
Summary
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Light-sheet fluorescence microscopy (LSFM) offers superior biological imaging but faces adoption challenges. This work clarifies LSFM principles to guide development and application for broader scientific use.

Area of Science:

  • Biological imaging
  • Biophysics
  • Neuroscience

Background:

  • Light-sheet fluorescence microscopy (LSFM) has broad applications in biology but has not replaced traditional methods.
  • The field's DIY approach and limited access to powerful systems hinder wider adoption.
  • Challenges associated with big image data in LSFM are significant.

Purpose of the Study:

  • To elucidate key developments in LSFM.
  • To define fundamental principles governing LSFM.
  • To guide the development and use of bespoke LSFM systems for biological research.

Main Methods:

  • Review and synthesis of LSFM principles and developments.
  • Identification of key decisions in designing and implementing LSFM systems.

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  • Focus on overcoming challenges in big image data management.

    • Main Results:

    • A simplified set of underlying principles for LSFM.
    • Clarification of design choices for bespoke LSFM systems.
    • Guidance for applying LSFM to diverse biological questions.

    Conclusions:

    • LSFM's potential is limited by accessibility and a lack of standardized principles.
    • Understanding core LSFM principles can facilitate wider adoption and application.
    • This work aims to democratize LSFM for broader biological discovery.