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Bacillus sphaericus LMG 22257 is physiologically suitable for self-healing concrete
Jianyun Wang1,2, Henk M Jonkers3, Nico Boon2
1Magnel Laboratory for Concrete Research, Ghent University, Technologiepark Zwijnaarde 904, 9052, Ghent, Belgium.
Applied Microbiology and Biotechnology
|April 3, 2017
Summary
Bacillus sphaericus, a spore-forming bacterium, shows promise for self-healing concrete. This ureolytic strain exhibits tolerance to alkaline conditions, calcium, and low temperatures, making it suitable for concrete applications.
Area of Science:
- Materials Science
- Microbiology
- Civil Engineering
Background:
- Concrete durability is a significant concern in civil engineering.
- Self-healing materials offer a sustainable solution to extend concrete infrastructure lifespan.
- Bacteria-based self-healing concrete utilizes microbial activity to repair cracks.
Purpose of the Study:
- To evaluate the suitability of Bacillus sphaericus for bacteria-based self-healing concrete.
- To assess the tolerance of B. sphaericus to alkaline pH, calcium, oxygen, and low temperatures.
Main Methods:
- Experimental evaluation of Bacillus sphaericus under various environmental conditions relevant to concrete.
- Assessment of bacterial growth, germination, and ureolytic activity in alkaline and calcium-rich environments.
- Testing bacterial performance at low temperatures (10°C) and evaluating mitigation strategies.
Main Results:
- Bacillus sphaericus demonstrated good tolerance to alkaline pH (optimal 7–9), with growth and germination occurring even at pH 10–11.
- The bacterium showed high calcium tolerance (0.9M Ca2+) without significant impact on ureolytic activity.
- B. sphaericus spores germinated and regained ureolytic activity at 10°C, albeit with retardation, which was mitigated by higher bacterial concentration and yeast extract.
Conclusions:
- Bacillus sphaericus is a suitable candidate for bacteria-based self-healing concrete due to its resilience.
- The ureolytic activity and tolerance profile of B. sphaericus support its application in diverse concrete environments.
- Further research can optimize conditions for enhanced performance in real-world concrete applications.