Interaction between catecholamines and vasoactive intestinal peptide in cultured astrocytes

¹Institute of Neurobiology, University of Göteborg, Sweden.

|March 1, 1988

Summary

Vasoactive intestinal peptide (VIP) receptors on rat astroglial cells activate cyclic AMP signaling, with regional differences. VIP also interacts with catecholamine pathways, potentially modulating astroglial responses to neurotransmitters.

Area of Science:

Neuroscience
Cell Biology
Biochemistry

Background:

Astroglial cells play crucial roles in brain function.
Vasoactive intestinal peptide (VIP) is a neuropeptide with diverse physiological effects.
Cyclic AMP (cAMP) is a key intracellular second messenger involved in signal transduction.

Purpose of the Study:

To investigate the presence and function of VIP receptors on cultured rat astroglial cells.
To characterize the role of VIP in modulating cyclic AMP (cAMP) levels in different brain regions.
To explore potential interactions between VIP and catecholamine signaling pathways in astrocytes.

Main Methods:

Primary astroglial cell cultures were established from newborn rat brain regions (cerebral cortex, striatum, hippocampus, brain stem).
Vasoactive intestinal peptide (VIP) and catecholamines (isoproterenol, norepinephrine) were used to stimulate cells.
Accumulation of cyclic AMP (cAMP) was measured as an indicator of receptor activation and signaling.

Main Results:

VIP receptors were identified on astroglial cells, mediating cAMP accumulation.
Regional differences in cAMP response to VIP were observed, with distinct patterns for nM and microM concentrations.
VIP dose-dependently inhibited isoproterenol-induced cAMP accumulation, suggesting interaction with beta-adrenergic pathways.
VIP did not inhibit norepinephrine-induced cAMP accumulation, consistent with interaction with alpha-adrenergic pathways.

Conclusions:

Cultured astroglial cells possess functional VIP receptors coupled to cAMP signaling.
VIP signaling in astrocytes exhibits regional specificity.
VIP signaling pathways interact with both alpha- and beta-adrenergic receptor-mediated cAMP signaling in astrocytes.
These interactions suggest VIP may modulate astroglial responses to catecholamines via intramembrane mechanisms.

Related Experiment Videos

Interaction between catecholamines and vasoactive intestinal peptide in cultured astrocytes

Related Concept Videos

Critical appraisal of the "one-day-per-percent" rule - A register-based observational study.

A randomized, triple-blinded controlled clinical study with a novel disease-modifying drug combination in equine lameness-associated osteoarthritis.

Nerve growth factor in the equine joint.

Patients with unilateral transfemoral amputation treated with a percutaneous osseointegrated prosthesis: a cost-effectiveness analysis.

Similar cost-utility for double- and single-bundle techniques in ACL reconstruction.

Long-term treatment with methylphenidate for fatigue after traumatic brain injury.

Dual MDM2 and PPARG regulatory axes mediate the antiepileptic effects of Gouteng: An Integration of Bioinformatics, Network Pharmacology, Machine Learning, Molecular Docking, and MD Simulations.

The Positive Feedback Interaction Between Mast Cell-Derived Tryptase and NETs Formation in the Pathogenesis of Sepsis-Associated Encephalopathy.

M3 muscarinic receptor antagonism attenuates cocaine-induced hippocampal oligodendroglial changes and relapse-like behavior in male mice.

Inferior olive-selective amplitude suppression and progressive dentato-cortical decoupling during haloperidol-induced parkinsonism: a longitudinal multi-unit activity study.

Digestion of perineuronal nets in the ventral CA1 suppresses methamphetamine-induced conditioned place preference acquisition by modulating PV interneuron activity in female mice.

Sex-specific differences in endocannabinoid regulation of cocaine-evoked dopamine in the medial nucleus accumbens shell.

AUT00206, a novel and selective Kv3.1/3.2 channel modulator, restores cognitive dysfunction and negative symptoms in an animal model for schizophrenia symptomatology.

Hypernatremia as the Initial Presentation of Osmotic Demyelination Syndrome: A Rare Case with Favorable Neurological Outcome.

Calycosin attenuates LPS-induced microglia inflammatory responses and microglia-mediated synaptic impairment via modulation of TLR4/MyD88/NF-κB signaling pathway.

Structure of NHE6 and its lipid-mediated interactions regulating endosomal pH.

Trigeminal Nerve Electrical Stimulation Attenuates Hypoxic-Ischemic Brain Injury by Regulating GLT-1-mediated Endoplasmic Reticulum Stress.

Divergent cortical-subcortical intrinsic neural timescales in first-episode drug-naïve or minimally treated schizophrenia.

Related Experiment Videos

Interaction between catecholamines and vasoactive intestinal peptide in cultured astrocytes

Area of Science:

Background:

Purpose of the Study:

Main Methods:

Main Results:

Conclusions:

Related Concept Videos

Related Articles