Nerve growth factor in the equine joint.
A Kendall1, S Nyström2, S Ekman1
1Division of Pathology, Pharmacology and Toxicology, Department of Biomedical Sciences and Veterinary Public Health, Swedish University of Agricultural Sciences, Box 7028, 750 07 Uppsala, Sweden.
Veterinary Journal (London, England : 1997)
|December 30, 2020
Summary
Nerve growth factor (NGF) is elevated in equine osteoarthritis and septic joints. NGF and its receptors are also present in cartilage, suggesting a role in equine joint disease.
Area of Science:
- Veterinary Medicine
- Biochemistry
- Immunology
Background:
- Nerve growth factor (NGF) plays roles in inflammation and pain signaling in humans and dogs.
- Osteoarthritis is prevalent in horses, but NGF's role in equine joints is unstudied.
- NGF is implicated in canine and human osteoarthritis synovial fluid.
Purpose of the Study:
- Quantify NGF in equine synovial fluid from healthy, septic, and osteoarthritic joints.
- Determine the localization of NGF and its receptors (TrkA, p75NTR) in healthy and osteoarthritic equine cartilage.
Main Methods:
- ELISA was used to measure NGF concentrations in synovial fluid.
- Immunohistochemistry was employed to examine NGF, TrkA, and p75NTR in articular cartilage.
Main Results:
- NGF was detected in equine synovial fluid and cartilage.
- Synovial fluid NGF concentrations were significantly higher in osteoarthritic and septic joints compared to healthy joints.
- Increased NGF and receptor staining was observed in osteoarthritic cartilage.
Conclusions:
- NGF is present in equine joints and elevated in osteoarthritis and septic inflammation.
- NGF and its receptors are localized in equine articular cartilage.
- Further research should investigate NGF precursor forms and downstream receptor signaling.
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