Related Experiment Videos
Gallbladder microbiota variability in Colombian gallstones patients
Ariel Antonio Arteta1, Hernan Carvajal-Restrepo, Miryan Margot Sánchez-Jiménez
1Faculty of Medicine - CES University, Medellín, Colombia. arteta.antonio@uces.edu.co.
Journal of Infection in Developing Countries
|April 4, 2017
Summary
Gallbladder stones harbor diverse bacteria, with Salmonella spp. identified in 3.3% of Colombian patients. Geographical factors influence gallbladder microbiota composition and potential Salmonella carrier states.
Area of Science:
- Microbiology
- Gastroenterology
- Infectious Diseases
Background:
- Gallstones are common and facilitate bacterial presence in the gallbladder, despite bile's natural antimicrobial properties.
- Salmonella spp. infection can lead to a carrier state where bacteria persist in the gallbladder without causing symptoms.
Purpose of the Study:
- To isolate and identify bacteria within gallstones from Colombian patients.
- To molecularly detect Salmonella spp. carriers among these patients.
Main Methods:
- Culturing of gallbladder tissue and bile from 149 patients with gallstones (cholecystectomy, no acute inflammation).
- DNA extraction and polymerase chain reaction (PCR) for the Salmonella spp. hilA gene.
Main Results:
- Bacterial cultures were positive in 19% of patients, with Pseudomonas spp., Klebsiella spp., and Proteus spp. most common in Medellín.
- Enterobacter cloacae and Raoultella terrigena were prevalent in Apartado.
- Salmonella spp. (hilA gene) was detected in 3.3% of patients, all females from the Medellín area.
Conclusions:
- Significant variability in gallbladder microbiota composition was observed.
- Geographical, ethnic, and environmental factors likely contribute to gallbladder microbiota differences and Salmonella carrier prevalence.