Bio-SELEX: A Strategy for Biomarkers Isolation Directly from Biological Samples
Juan David Ospina-Villa1, Valentina Restrepo-Cano1, Miryan Margot Sánchez-Jiménez1
1Instituto Colombiano de Medicina Tropical-ICMT, Universidad CES, Sabaneta 055450, Colombia.
Methods and Protocols
|November 21, 2023
Summary
Bio-SELEX is a novel method for discovering disease biomarkers using aptamers and mass spectrometry. This technique aids in diagnosing infectious and non-infectious diseases for better healthcare outcomes.
Area of Science:
- Biotechnology
- Molecular Biology
- Analytical Chemistry
Background:
- Accurate biomarker discovery is crucial for diagnosing and monitoring diseases.
- Existing methods may lack specificity or efficiency in complex biological samples.
- Novel approaches are needed for comprehensive disease diagnostics.
Purpose of the Study:
- To introduce and detail the Bio-SELEX methodology for novel biomarker discovery.
- To highlight the application of Bio-SELEX in identifying biomarkers for various diseases.
- To showcase the potential of Bio-SELEX in advancing diagnostics and personalized medicine.
Main Methods:
- Bio-SELEX integrates Traditional SELEX (Systematic Evolution of Ligands by Exponential Enrichment) for aptamer generation.
- A Pull-Down assay using selected aptamers isolates target biomarkers from biological samples.
- Mass spectrometry identifies and quantifies the captured biomarkers for diagnostic insights.
Main Results:
- The study demonstrates Bio-SELEX's capability to discover specific nucleic acid aptamers.
- The method successfully isolates and identifies target biomarkers from complex matrices.
- Quantitative data obtained aids in disease diagnosis and monitoring.
Conclusions:
- Bio-SELEX offers a powerful and versatile platform for biomarker discovery across infectious and non-infectious diseases.
- This approach facilitates early disease detection and the development of targeted therapies.
- Bio-SELEX enhances understanding of disease biology and opens new healthcare avenues.
