Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A DNAzyme Feedback Amplification Strategy for Biosensing.

Meng Liu1,2, Qiang Zhang2, Dingran Chang1

  • 1Department of Biochemistry and Biomedical Sciences and Chemistry & Chemical Biology, McMaster University, 1280 Main Street West, Hamilton, ON, L8S 4K1, Canada.

Angewandte Chemie (International Ed. in English)
|April 4, 2017
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A Portable Colorimetric Device for Rapid Bacterial Detection with Cleavable Functional Nucleic Acid Probes for A Common Bacterial Endoribonuclease.

Angewandte Chemie (International ed. in English)·2026
Same author

Biomolecular Condensates as Protein Degradation Tools for Intracellular Targets.

Nature communications·2026
Same author

Engineering High-Performance Aptamers via Optimized SELEX and Structure-Guided Dimer Assembly for Clinical Malaria Diagnostics.

Analytical chemistry·2026
Same author

A general strategy to enhance aptamer affinity by suppressing dissociation through symmetric assembly.

Nucleic acids research·2026
Same author

Translating a miRNA Signal into Physical Immunomodulation via Programmed DNA Network Assembly on Mitochondria.

Journal of the American Chemical Society·2026
Same author

A Real-Time Antifouling Multivalent Aptamer Platform for Wash-Free Electrochemical Detection of Low-Abundance Biomarkers in Human Plasma.

Angewandte Chemie (International ed. in English)·2026

We developed DNAzyme feedback amplification (DFA), a novel method using rolling-circle amplification and DNAzymes for exponential DNA amplification. This technique significantly enhances biosensing sensitivity for targets like microRNA and bacteria.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Biotechnology

Background:

  • Signal amplification is crucial for sensitive biosensing.
  • Existing methods often face limitations in sensitivity and complexity.

Purpose of the Study:

  • To introduce a novel signal amplification strategy called DNAzyme feedback amplification (DFA).
  • To demonstrate the application of DFA in sensitive biosensing of microRNA and bacteria.

Main Methods:

  • DFA combines rolling-circle amplification (RCA) with an RNA-cleaving DNAzyme (RCD).
  • Two DNA complexes initiate a self-sustaining reaction circuit for exponential amplification.
  • RCA produces RCD elements that cleave a substrate, triggering further RCA initiation.

Main Results:

Keywords:
DNAzymesRNA cleavagebiosensorsfeedback amplificationrolling-circle amplification

Related Experiment Videos

  • Achieved exponential DNA amplification through an autonomous reaction circuit.
  • Demonstrated DFA's versatility in detecting a specific microRNA sequence.
  • Successfully detected a bacterium using the DFA approach.
  • Reported sensitivity improvements of 3-6 orders of magnitude compared to conventional methods.

Conclusions:

  • DFA is a powerful and versatile signal amplification strategy for biosensing.
  • The method offers significant improvements in sensitivity for detecting various biological targets.
  • DFA holds promise for developing next-generation diagnostic tools.