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Updated: Jul 27, 2026

Surface Potential Measurement of Bacteria Using Kelvin Probe Force Microscopy
Published on: November 28, 2014
Reconstruction of Kelvin probe force microscopy image with experimentally calibrated point spread function
Fei Lan1, Minlin Jiang1, Quan Tao1
1Department of Electrical and Computer Engineering, University of Pittsburgh, Pittsburgh, Pennsylvania 15261, USA.
Kelvin probe force microscopy (KPFM) images are often blurred. This study calibrates the KPFM system
Area of Science:
- Surface Science
- Nanotechnology
- Materials Science
Background:
- Kelvin probe force microscopy (KPFM) images can be difficult to interpret due to blurring.
- This blurring arises from the long-range nature of electrostatic forces detected by KPFM.
- The KPFM imaging process is modeled as a convolution of the true surface potential (SP) with the system's point spread function (PSF).
Purpose of the Study:
- To develop a method for experimentally calibrating the KPFM system's point spread function (PSF).
- To improve the accuracy of KPFM surface potential (SP) measurements by addressing image blurring.
- To enhance the fidelity of SP signals and reduce noise in KPFM imaging.
Main Methods:
- Experimental calibration of the KPFM system's point spread function (PSF), considering probe shape and experimental parameters.
- Application of a nonlinear reconstruction algorithm utilizing total variation (TV) regularization.
- Reversing the blurring effect caused by the PSF during KPFM imaging.
Main Results:
- An experimentally calibrated PSF that is more accurate than simulated ones.
- Successful reversal of KPFM image blurring.
- Significant reduction in noise and improvement in the fidelity of surface potential (SP) signals.
Conclusions:
- Experimental PSF calibration provides a more accurate representation for KPFM systems.
- The developed method effectively restores true surface potential (SP) distributions from blurred KPFM images.
- This approach enhances the interpretability and reliability of KPFM measurements for materials analysis.
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