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Whole-Cell Recording in the Awake Brain
1Center for Cognition and Sociality, Institute for Basic Science, Daejeon 34141, Republic of Korea; leedoyun@ibs.re.kr leea@janelia.hhmi.org.
Cold Spring Harbor Protocols
|April 5, 2017
Summary
Intracellular recording in awake, behaving animals is advancing. New methods allow precise measurement and manipulation of neuronal activity to understand brain computations.
Area of Science:
- Neuroscience
- Cellular Biology
- Electrophysiology
Background:
- Intracellular recording is crucial for studying neural mechanisms of behavior.
- The technique is sensitive to mechanical disturbances, posing challenges for in vivo applications.
- Previous methods were limited in awake, freely moving subjects.
Purpose of the Study:
- To review recent advancements in intracellular recording techniques for behaving animals.
- To highlight applications in measuring and manipulating neuronal membrane potential.
- To connect these methods to understanding neuronal computations.
Main Methods:
- Summarizing recent technological and methodological improvements in intracellular recording.
- Discussing strategies to overcome sensitivity to movement artifacts.
- Reviewing techniques for real-time data acquisition and stimulation.
Main Results:
- Demonstration of successful intracellular recordings in awake, behaving, and freely moving animals.
- Advancements enabling precise measurement of membrane potential dynamics.
- New possibilities for manipulating neuronal activity in conjunction with behavior.
Conclusions:
- Intracellular recording in behaving animals is becoming more robust and versatile.
- These techniques provide powerful tools for dissecting neural circuits underlying complex behaviors.
- Future research can leverage these advances to further elucidate neuronal computations.