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Generating Recombinant Vesicular Stomatitis Viruses for Use as Vaccine Platforms
John B Ruedas1, John H Connor2
1Department of Microbiology and National Emerging Infectious Disease Laboratory, Boston University School of Medicine, Boston, MA, USA.
Methods in Molecular Biology (Clifton, N.J.)
|April 5, 2017
Summary
Vesicular stomatitis virus (VSV) shows promise as a vaccine platform. Researchers can now create recombinant VSV vaccines targeting various pathogens using plasmid-based methods.
Area of Science:
- Virology
- Vaccinology
- Molecular Biology
Background:
- Vesicular stomatitis virus (VSV) possesses unique characteristics suitable for vaccine development.
- Advancements in plasmid-based technologies enable the creation of recombinant VSV expressing foreign glycoproteins.
Purpose of the Study:
- To provide an overview of VSV as a vaccine platform.
- To detail the methodology for generating recombinant VSV from plasmids.
Main Methods:
- Utilizing plasmid-based systems for recombinant VSV generation.
- Engineering VSV to express heterologous viral glycoproteins.
Main Results:
- Demonstration of VSV's potential as a versatile vaccine vector.
- Establishment of a protocol for producing recombinant VSV vaccine candidates.
Conclusions:
- Recombinant VSV offers a flexible platform for developing vaccines against diverse human pathogens.
- Plasmid-based generation facilitates the rapid development of VSV-based vaccine candidates.