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Bovine leukocyte interferon: characterization and large-scale production
K L Jacobsen1, G A Rockwood, M Abolhassani
1Department of Large Animal Medicine, College of Veterinary Medicine, University of Georgia, Athens 30602.
Journal of Interferon Research
|April 1, 1988
Summary
High-titer natural bovine leukocyte interferon (IFN) was successfully produced using Sendai virus. This bovine interferon demonstrated stability at cold temperatures and 37°C, showing potential for clinical applications in cattle.
Area of Science:
- Veterinary Virology
- Immunology
- Biotechnology
Background:
- Bovine leukocyte interferon (IFN) is crucial for antiviral defense in cattle.
- Efficient and scalable production methods are needed for clinical applications.
Purpose of the Study:
- To develop a high-titer production system for natural bovine leukocyte interferon.
- To assess the stability and characteristics of the produced bovine IFN.
Main Methods:
- Utilized Sendai virus as an inducer for bovine leukocyte interferon production.
- Optimized inducer concentration (300 HA/ml) and incubation time (12 h).
- Assessed antiviral activity stability under various temperature and pH conditions, and after enzymatic treatment.
Main Results:
- Achieved high titers of bovine leukocyte interferon (15,314 U/ml).
- Demonstrated complete stability for 30 days at 4°C, -20°C, and -70°C, and 96 hours at 37°C.
- Showed partial loss of activity at 56°C for 15 min, complete loss with trypsin, and no effect with pH 2.0 dialysis. Inhibited bovine kidney cell growth.
Conclusions:
- A robust system for producing clinically relevant quantities of bovine leukocyte interferon has been established.
- The produced bovine IFN exhibits favorable stability, supporting its potential therapeutic use in cattle.
- Further research can explore its efficacy in treating viral infections in cattle.