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Published on: February 14, 2019
Synthetic Glycoforms Reveal Carbohydrate-Dependent Bioactivity of Human Saposin D
Christopher G F Graf1, Christian Schulz1, Marina Schmälzlein1
1Bioorg. Chemie, Gebäude NWI, Universität Bayreuth, 95440, Bayreuth, Germany.
Abstract:
The main glycoforms of the hydrophobic lysosomal glycoprotein saposin D (SapD) were synthesized by native chemical ligation. An approach for the challenging solid-phase synthesis of the fragments was developed. Three SapD glycoforms were obtained following a general and robust refolding and purification protocol. A crystal structure of one glycoform confirmed its native structure and disulfide pattern. Functional assays revealed that the lipid-binding properties of three SapD glycoforms are highly affected by the single sugar moiety of SapD showing a dependency of the size and the type of N-glycan.
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