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Simultaneous Triple Imaging with Two PARASHIFT Probes: Encoding Anatomical, pH and Temperature Information using
Katie-Louise N A Finney1, Alice C Harnden1, Nicola J Rogers1
1Department of Chemistry, Durham University, South Road, Durham, DH1 3LE, UK.
Chemistry (Weinheim an Der Bergstrasse, Germany)
|April 6, 2017
Summary
Researchers developed a new probe using lanthanide complexes to simultaneously measure temperature and pH. This advancement allows for triple imaging, providing insights into biological conditions in organs like the liver and kidneys.
Area of Science:
- Inorganic Chemistry
- Analytical Chemistry
- Biomedical Imaging
Background:
- Lanthanide(III) complexes can report temperature via chemical shift.
- Modifying these complexes allows for pH sensing alongside temperature.
- Developing sensitive probes is crucial for in vivo diagnostics.
Purpose of the Study:
- To create a novel probe for simultaneous temperature and pH reporting.
- To demonstrate the capability of simultaneous triple imaging using lanthanide complexes.
- To validate the probe's utility in measuring physiological conditions in organs.
Main Methods:
- Synthesized lanthanide(III) complexes with a phosphonate group for pH sensing.
- Utilized paramagnetically shifted resonances for signal detection.
- Performed parallel spectral imaging experiments to differentiate signals.
- Deconvoluted temperature and pH dependencies of thulium and dysprosium signals.
Main Results:
- Achieved simultaneous triple imaging of water, thulium, and dysprosium signals.
- Demonstrated signal separation exceeding 160 ppm.
- Successfully deconvoluted temperature and pH dependencies.
- Measured temperature and pH in liver, kidney, and bladder tissues.
Conclusions:
- The developed probe enables simultaneous, sensitive measurement of temperature and pH.
- This technique allows for in vivo physiological monitoring in multiple organs.
- Offers a promising tool for advanced biomedical imaging and diagnostics.