Related Experiment Videos
Specific binding of [3H]retinoids to cellular retinoid-binding proteins
M Ninomiya1, M Suganuma, N S Paik
1National Cancer Center Research Institute, Tokyo, Japan.
FEBS Letters
|June 20, 1988
Abstract:
The specific binding of [3H]retinoids to cellular retinoid-binding proteins was measured directly by the cold acetone filtration method. After incubation of purified cellular retinoid-binding proteins with [3H]retinoids with or without competitors for 2-4 h, bound ligands were separated from free by filtration using cold acetone. Nonspecific binding of the ligands was reduced sufficiently to allow measurement of specific binding of [3H]retinoids to cellular retinoid-binding proteins. This method has the advantages of being rapid and practical and giving reproducible results.