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Related Experiment Videos

Methods for Quantitative Creatinine Determination.

John F Moore1, J Daniel Sharer1

  • 1Department of Genetics, University of Alabama at Birmingham, Birmingham, Alabama.

Current Protocols in Human Genetics
|April 7, 2017
PubMed
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Accurate creatinine measurement is vital for assessing kidney function and drug levels. This review covers the Jaffe method, HPLC, and LC-MS/MS, recommending LC-MS/MS for standardizing creatinine quantification.

Area of Science:

  • Clinical Chemistry
  • Analytical Chemistry
  • Biomedical Engineering

Background:

  • Accurate creatinine measurement is crucial for evaluating kidney function and monitoring drug levels.
  • The Jaffe method, based on alkaline creatinine-picric acid complex color formation, is widely used but susceptible to interference.
  • Interfering substances in serum and urine can affect the accuracy of Jaffe creatinine measurements.

Purpose of the Study:

  • To review and compare various methods for creatinine quantification.
  • To highlight the limitations of the Jaffe method and introduce advanced techniques.
  • To discuss the standardization of creatinine measurements.

Main Methods:

  • Summary of the basic Jaffe method and its automated modifications.
Keywords:
HPLCLC-MS/MScreatinineglomerular filtration ratepicric acid

Related Experiment Videos

  • Description of High-Performance Liquid Chromatography (HPLC) for creatinine separation and UV quantification.
  • Detailed explanation of Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS) using stable isotope dilution.

    • Main Results:

    • The Jaffe method is prone to interference from common serum and urine compounds.
    • HPLC offers improved separation of creatinine from contaminants.
    • LC-MS/MS provides reliable creatinine quantification with stable isotope dilution, enabling standardization.

    Conclusions:

    • Advanced methods like HPLC and LC-MS/MS overcome the limitations of the Jaffe method.
    • LC-MS/MS is recommended for standardizing all quantitative creatinine assays.
    • Reliable creatinine quantification is essential for accurate clinical diagnostics and therapeutic drug monitoring.