Related Experiment Video
Updated: Jun 17, 2026
In vivo Dual Substrate Bioluminescent Imaging
Published on: October 11, 2011
Luciferase-Rose Bengal conjugates for singlet oxygen generation by bioluminescence resonance energy transfer
Seonghoon Kim1, HyeongChan Jo2, Mijeong Jeon2
1Harvard Medical School and Wellman Center for Photomedicine, Massachusetts General Hospital, USA. syun@hms.harvard.edu and Graduate School of Nanoscience and Technology, Korea Advanced Institute of Science and Technology, Korea.
Abstract:
Conjugates of Rose Bengal and Renilla luciferase generated singlet oxygen upon binding with coelenterazine via bioluminescence resonance energy transfer (BRET). Since the applications of conventional PDT have been limited to superficial lesions due to the limited light penetration in tissue, BRET activated PDT which does not require external light illumination may overcome the limitations of conventional PDT.
