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Purification and Characterization of the Bacterial Flagellar Basal Body from Salmonella enterica
1Department of Life Sciences, Prefectural University of Hiroshima, 562 Nanatsuka, Shobara, Hiroshima, 727-0023, Japan. aizawa@pu-hiroshima.ac.jp.
Methods in Molecular Biology (Clifton, N.J.)
|April 9, 2017
Abstract:
The bacterial flagellum is a motility organelle. The flagellum is composed of three main structures: the basal body as a rotary engine embedded in the cellular membranes and cell wall, the long external filament that acts as a propeller, and the hook acting as a universal joint that connects them. I describe protocols for the purification of the filament and hook-basal body from Salmonella enterica serovar Typhimurium.