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Isolation and Characterization of a Novel Phage SaGU1 that Infects Staphylococcus aureus Clinical Isolates from

Yuzuki Shimamori1,2, Ajeng K Pramono3, Tomoe Kitao1

  • 1Department of Microbiology, Graduate School of Medicine, Gifu University, 1-1 Yanagido, Gifu, Gifu, 501-1194, Japan.

Current Microbiology
|February 27, 2021
PubMed

Insights

A novel bacteriophage, SaGU1, shows promise for treating Staphylococcus aureus infections in atopic dermatitis (AD). This phage effectively targets S. aureus strains from AD skin, offering a potential alternative to antibiotics.

Area of Science:

  • Microbiology
  • Virology
  • Dermatology

Background:

  • Staphylococcus aureus is a common skin colonizer that can cause atopic dermatitis (AD).
  • Antibiotic resistance necessitates alternative treatments for S. aureus infections.
  • Phage therapy presents a promising alternative to conventional antibiotic treatments.

Purpose of the Study:

  • To characterize the bacteriophage SaGU1 as a potential therapeutic agent against S. aureus.
  • To evaluate SaGU1's efficacy in targeting S. aureus strains from atopic dermatitis patients.
  • To assess the safety and host range of SaGU1 for potential phage therapy applications.

Main Methods:

  • Screening of bacteriophages from sewage samples.
  • Genomic sequencing and bioinformatics analysis of bacteriophage SaGU1.
  • Electron microscopy for morphology determination.
  • Lytic activity, stability, and host range assays.

Main Results:

  • SaGU1 possesses a 140,909 bp genome with 225 protein-coding genes and no toxic or antibiotic resistance genes.
  • Electron microscopy identified SaGU1 as belonging to the Myoviridae family.
  • SaGU1 demonstrated stability under physiological and acidic conditions.
  • SaGU1 exhibited broad-spectrum lytic activity against S. aureus clinical isolates from AD patients, sparing Staphylococcus epidermidis.

Conclusions:

  • Bacteriophage SaGU1 is a heat-stableMyoviridae phage with a broad host range against S. aureus relevant to atopic dermatitis.
  • SaGU1 lacks undesirable genes, indicating its safety for therapeutic use.
  • SaGU1 represents a promising candidate for developing phage therapy against S. aureus-associated atopic dermatitis.

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