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Updated: Jul 11, 2026

Laboratory Techniques Used to Maintain and Differentiate Biotypes of Vibrio cholerae Clinical and Environmental Isolates
Published on: May 30, 2017
Chemotactic Behaviors of Vibrio cholerae Cells
Ikuro Kawagishi1,2, So-Ichiro Nishiyama3,4
1Department of Frontier Bioscience, Hosei University, Kajino-cho, Koganei, Tokyo, 184-8584, Japan. ikurok@hosei.ac.jp.
Abstract:
Vibrio cholerae, the causative agent of cholera, swims in aqueous environments with a single polar flagellum. In a spatial gradient of a chemical, the bacterium can migrate in "favorable" directions, a property that is termed chemotaxis. The chemotaxis of V. cholerae is not only critical for survival in various environments and but also is implicated in pathogenicity. In this chapter, we describe how to characterize the chemotactic behaviors of V. cholerae: these methods include swarm assay, temporal stimulation assay, capillary assay, and receptor methylation assay.
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