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Magnetic separate "turn-on" fluorescent biosensor for Bisphenol A based on magnetic oxidation graphene
Liu-Yin Hu1, Cheng-Gang Niu1, Xiao-Yu Wang1
1College of Environmental Science Engineering, Key Laboratory of Environmental Biology Pollution Control, Ministry of Education, Hunan University, Changsha 410082, China.
Abstract:
Bisphenol A (BPA) is commonly considered to cause a health hazard to wildlife and humans, acting as an exogenous estrogen. Herein, a magnetic separate "turn-on" fluorescent method for the detection of BPA was proposed based on fluorescence resonance energy transfer (FRET) between fluorescein-labeled BPA aptamer and magnetic oxidation graphene (MGO). At different concentrations of BPA, the fluorescence intensity of the sensing system was varied. The detection limit of 0.071ng/mL was obtained with the linear range of 0.2-10ng/mL. The biosensor exhibited excellent anti-interference ability and selectivity in actual water samples.