Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Development and translation of biodegradable metal stents: from heart to brain.

Regenerative biomaterials·2026
Same author

PLANeT: Understanding and leveraging the genome of land plants for a sustainable future.

Cell·2026
Same author

Time-Variant Image Inpainting via Interactive Distribution Transition Estimation.

IEEE transactions on image processing : a publication of the IEEE Signal Processing Society·2026
Same author

A structural variation-driven evolutionary history of upland cotton.

Science China. Life sciences·2026
Same author

Tuning Xylan Polymerisation Enhanced Fibre Digestibility Without Biomass Loss in Sheepgrass (Leymus chinensis).

Plant biotechnology journal·2026
Same author

Empowering drug innovation through fundamental RNA research in China.

Nature chemical biology·2026
Same journal

Better breeding leveraging more biology.

Trends in plant science·2026
Same journal

Women in plant science around the world.

Trends in plant science·2026
Same journal

Bilateral symmetry genes: If they exist, how would we know?

Trends in plant science·2026
Same journal

From xylem atlases to developmental continuity in forestry.

Trends in plant science·2026
Same journal

Small peptides guard the gate of plant immunity.

Trends in plant science·2026
Same journal

Phosphorylation blues: Cracking the phototropin phosphocode.

Trends in plant science·2026
See all related articles

Related Experiment Video

Updated: Dec 16, 2025

Methodology for Accurate Detection of Mitochondrial DNA Methylation
12:11

Methodology for Accurate Detection of Mitochondrial DNA Methylation

Published on: May 20, 2018

13.8K

Context and Complexity: Analyzing Methylation in Trinucleotide Sequences.

Xianwei Song1, Xiaofeng Cao2

  • 1State Key Laboratory of Plant Genomics and National Center for Plant Gene Research, CAS Center for Excellence in Molecular Plant Sciences, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.

Trends in Plant Science
|April 11, 2017
PubMed
Summary
This summary is machine-generated.

Plant cytosine methylation, crucial for gene regulation, shows significant variation in CHG/CHH contexts. This heterogeneity is linked to the function of chromomethyltransferases in maintaining DNA methylation patterns.

More Related Videos

Targeted DNA Methylation Analysis by Next-generation Sequencing
08:38

Targeted DNA Methylation Analysis by Next-generation Sequencing

Published on: February 24, 2015

37.8K
DNA Methylation: Bisulphite Modification and Analysis
12:34

DNA Methylation: Bisulphite Modification and Analysis

Published on: October 21, 2011

106.1K

Related Experiment Videos

Last Updated: Dec 16, 2025

Methodology for Accurate Detection of Mitochondrial DNA Methylation
12:11

Methodology for Accurate Detection of Mitochondrial DNA Methylation

Published on: May 20, 2018

13.8K
Targeted DNA Methylation Analysis by Next-generation Sequencing
08:38

Targeted DNA Methylation Analysis by Next-generation Sequencing

Published on: February 24, 2015

37.8K
DNA Methylation: Bisulphite Modification and Analysis
12:34

DNA Methylation: Bisulphite Modification and Analysis

Published on: October 21, 2011

106.1K

Area of Science:

  • Plant molecular biology
  • Epigenetics
  • Genomics

Background:

  • Cytosine methylation is a key epigenetic modification in plants, occurring in CG, CHG, and CHH sequence contexts.
  • Distinct molecular pathways are responsible for maintaining methylation in each of these sequence contexts.
  • Understanding methylation patterns is crucial for deciphering gene regulation and plant development.

Purpose of the Study:

  • To investigate the heterogeneity of cytosine methylation levels in the CHG and CHH sequence contexts in plants.
  • To explore the association between this methylation heterogeneity and the function of chromomethyltransferases.

Main Methods:

  • Analysis of DNA methylation patterns across different sequence contexts (mCG, mCHG, mCHH).
  • Utilizing recent publication data on methylation heterogeneity.
  • Correlating methylation levels with the known functions of chromomethyltransferases.

Main Results:

  • Substantial heterogeneity in cytosine methylation levels was observed specifically within the CHG and CHH trinucleotide contexts.
  • This observed heterogeneity is functionally associated with the role of chromomethyltransferases in maintaining DNA methylation.

Conclusions:

  • Plant DNA methylation exhibits context-specific heterogeneity, particularly in non-CG sites.
  • Chromomethyltransferases play a significant role in establishing and maintaining methylation patterns in the CHG/CHH contexts, contributing to the observed heterogeneity.