Related Experiment Videos
Reference values for fasting serum resistin in healthy children and adolescents
Ulrik Lausten-Thomsen1, Michael Christiansen2, Paula Louise Hedley3
1The Children's Obesity Clinic, Department of Paediatrics, Copenhagen University Hospital Holbæk, Holbæk, Denmark; Department of Clinical Medicine, Copenhagen University, Copenhagen, Denmark.
Insights
Serum resistin levels in children and adolescents vary by sex and correlate with BMI. These findings establish pediatric reference ranges for resistin, aiding in disease risk assessment.
Area of Science:
- Endocrinology
- Pediatric Health
- Biomarkers
Background:
- Resistin is an inflammatory hormone linked to chronic diseases like insulin resistance.
- Pediatric reference levels for serum resistin are crucial for risk stratification.
- Understanding resistin in children is vital for interpreting individual concentrations.
Purpose of the Study:
- To establish pediatric reference ranges for serum resistin concentrations.
- To investigate the correlation between resistin levels, BMI, age, and sex in healthy children.
- To provide data for improved risk assessment in pediatric populations.
Main Methods:
- Quantified fasting serum resistin in 1191 healthy Danish schoolchildren (ages 6-18).
- Utilized Human Resistin ELISA Development kit (R&D Systems).
- Analyzed correlations with relative BMI, age, and sex.
Main Results:
- Median resistin: 8.93 ng/mL (boys) and 10.42 ng/mL (girls).
- Resistin correlated with relative BMI in both sexes (p=0.02 boys, p<0.0001 girls).
- Age-correlated increase observed in girls (p=0.02), not in boys (p=0.35).
Conclusions:
- Fasting serum resistin concentrations differ between sexes in children and adolescents.
- Resistin levels correlate with sex- and age-adjusted BMI.
- In girls, resistin also correlates with age, highlighting sex-specific patterns.
Background:
Resistin is a hormone, mainly produced in macrophages and monocytes, believed to play an important role in the inflammatory response. It has been linked to several chronic diseases such as heart failure, inflammatory bowel disease, and insulin resistance. Pediatric reference levels are needed for the risk stratification and interpretation of individual serum resistin concentrations.
Methods:
A total of 1191 healthy, non-obese Danish schoolchildren (727 girls) aged 6-18years (median 11.9) were included. Fasting serum resistin concentrations were quantitated by Human Resistin ELISA Development kit, Duo Set (R&D Systems) following optimization.
Results:
The overall median resistin concentration was 8.93ng/mL (interquartile range (IQR): 6.19-13.33, range 1.57-35.84) in boys and 10.42ng/mL (IQR: 7.25-15.68, range 1.60-44.00) in girls. The resistin concentration correlated to relative BMI in both boys (p=0.02) and girls (p<0.0001). Percentiles for each age group were calculated alongside smoothed percentile curves and an age correlated increase was demonstrated, albeit only in girls (p=0.02) and not in boys (p=0.35).
Conclusion:
Fasting serum resistin concentrations differ between sexes in healthy children and adolescents and are correlated both with the sex- and age adjusted BMI, and in girls to age.