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Technological and Theoretical Aspects for Testing Electroporation on Liposomes.
Agnese Denzi1, Elena Della Valle2, Gianluca Esposito2
1Center for Life Nano Science@Sapienza, Istituto Italiano di Tecnologia, 00161 Rome, Italy; Department of Information Engineering, Electronics and Telecommunication (DIET), University of Rome "La Sapienza", 00184 Rome, Italy.
Biomed Research International
|April 11, 2017
Summary
This study presents a novel 10-nanosecond pulsed electric field (nsPEF) system for electroporating nanometer liposomes. The system facilitates simultaneous liposome and cell membrane electroporation for enhanced drug delivery.
Area of Science:
- Biomedical Engineering
- Nanotechnology
- Drug Delivery Systems
Background:
- Nanometer liposomes are emerging as effective nanocarriers for drug delivery.
- Nanoelectroporation offers a promising technique for enhancing drug uptake by cells.
- Simultaneous electroporation of liposomes and cell membranes can improve drug release and cellular internalization.
Purpose of the Study:
- To design and characterize a 10-nanosecond pulsed electric field (nsPEF) exposure system for liposome electroporation.
- To evaluate the efficiency of the nsPEF system under varying solution conductivities.
- To investigate the influence of device performance on liposome electroporation through microdosimetric simulations.
Main Methods:
- Design and characterization of a nsPEF exposure system using an electroporation cuvette with a 1 mm electrode gap.
- Evaluation of system efficiency by varying solution conductivity (0.25 to 1.6 S/m).
- Microdosimetric simulations of liposomes (200 and 400 nm) with varying conductivities to determine poration voltage.
Main Results:
- The study successfully designed and characterized a nsPEF system for liposome electroporation.
- System efficiency was evaluated across a range of solution conductivities.
- Simulations identified the necessary voltages for porating liposomes of different sizes and conductivities.
Conclusions:
- The developed nsPEF system is suitable for liposome electroporation.
- Understanding the interplay between device parameters and liposome properties is crucial for optimizing drug delivery.
- This technology holds potential for advancing nanoelectroporation-based drug delivery systems.