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Updated: Jul 16, 2026

Enzyme-linked Immunospot Assay (ELISPOT): Quantification of Th-1 Cellular Immune Responses Against Microbial Antigens
Published on: November 24, 2010
[Perceptions about the elisa test of people diagnosed at the aids stage]
Paola Carrasco A1, Alejandra X Araya G2, Paula Vega1
1Escuela de Enfermería, Facultad de Medicina, Pontificia Universidad Católica de Chile, Santiago, Chile.
Background:
The delay in the diagnosis of AIDS results in higher treatment costs.
Aim:
To reveal the experiences of people who were diagnosed in the AIDS stage about the access to the ELISA test.
Material And Methods:
In depth interviews were carried out to 15 participants from public hospitals who were in the AIDS stage at the moment of the diagnosis. The main questions asked were about the motivations to take the test, the barriers found and the help received from the health care personnel. All interviews were recorded and analyzed according to Kripperdorff.
Results:
The three categories that emerged were the motivations to take the test, the facilitators found and the difficulties to access to the test. The main motivation was a condition of vulnerability due to the suspicion or certainty of being infected. The main facilitator was the sensation of being accepted and not discriminated. The main difficulties were the fear of having a positive test and of being discriminated and the lack of information.
Conclusions:
Knowing these experiences will help to improve the early detection of HIV infections.
Related Concept Videos
Enzyme-Linked Immunosorbent Assay
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen. Enzyme-substrate reaction allows the antigen to be visualized or quantified.
Sexually Transmitted Infections

