Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Studying microstructure and microstructural changes in plant tissues by advanced diffusion magnetic resonance imaging

Darya Morozov1, Iris Tal2, Odelia Pisanty2

  • 1School of Chemistry, The Sackler Faculty of Exact Sciences, and.

Journal of Experimental Botany
|April 12, 2017
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Navigating genetic redundancy in plant genomes: insights for research and breeding.

Trends in plant science·2026
Same author

Targeting redundant gene families: A multiplexed, tissue-specific CRISPR toolbox for Arabidopsis genetic screens.

Cell reports·2026
Same author

Quantifying Transmembrane Water Exchange by Diffusion NMR Methods: From Yeast Cells to Optic Nerve Ex Vivo.

NMR in biomedicine·2026
Same author

mTACT: A cell type-specific transportome-scale amiRNA toolbox to overcome functional redundancy in Arabidopsis.

Plant physiology·2025
Same author

Intramolecular hydrogen-bonding motifs in the 14-oxymorphinan opioids: An experimental and computational study.

European journal of medicinal chemistry·2025
Same author

Pillar[n]arenes versus cyclodextrins as supramolecular carriers for improved bioavailability: Idebenone as a model drug.

International journal of pharmaceutics·2025

Magnetic resonance imaging (MRI) non-invasively visualizes plant microstructure and adventitious root formation in tomato. Advanced diffusion MRI methods reveal cellular changes during root development, offering new insights into plant growth.

Area of Science:

  • Plant Biology
  • Biophysics
  • Imaging Science

Background:

  • Plants, as sessile organisms, rely on environmental responses for growth and development, primarily through meristematic activity.
  • Understanding post-embryonic plant development, especially lateral adventitious root formation, requires advanced visualization techniques.
  • Non-invasive methods to study plant microstructure and organ initiation are crucial for plant science research.

Purpose of the Study:

  • To characterize hypocotyl microstructure in tomato using advanced magnetic resonance imaging (MRI) techniques.
  • To investigate microstructural changes during lateral adventitious root development non-invasively.
  • To assess the utility of relaxation-based and diffusion MRI for studying plant organogenesis.

Main Methods:

Keywords:
Adventitious rootsSolanum lycopersicum.auxindiffusion MRI (DWI)diffusion tensor imaging (DTI)double-pulsed-field-gradient (d-PGSE) MRIhypocotyl structuremagnetic resonance imaging (MRI)microstructureplant developmentq-space diffusion MRI (QSI)tomato

Related Experiment Videos

  • Utilized relaxation-based and advanced diffusion MRI (DTI, QSI, d-PFG) at 14.1 T to analyze tomato hypocotyls.
  • Employed higher in-plane resolution for relaxation-based MRI, noting trade-offs with signal-to-noise ratio.
  • Applied diffusion MRI techniques to probe water diffusion anisotropy and cellular dimensions within the vascular cylinder.

    • Main Results:

    • Relaxation-based MRI provided better contrast at higher resolutions, albeit with reduced signal-to-noise.
    • Diffusion MRI demonstrated highly anisotropic water diffusion within the vascular cylinder.
    • QSI and d-PFG MRI indicated cell sizes of 6-10 μm in the vascular cylinder, capturing reorganization during adventitious root formation.

    Conclusions:

    • MRI and diffusion MRI are effective non-invasive tools for studying plant microstructural features.
    • These imaging methods enable the observation of microstructural dynamics associated with adventitious root formation.
    • The study highlights MRI's potential for advancing research in plant development and organogenesis.