Changes of c-mos expression in response to 2-O-tetradecanoylphorbol-13-acetate in undifferentiated teratocarcinoma cells

Treatment of the cells of the 311 cell line, a pluripotent mouse teratocarcinoma cell line, with 12-O-tetradecanoylphorbol-13-acetate (TPA) modulates c-mos expression. Transient suppression of 6.1 and 4.6 kilobases (kb) transcripts and activation of 1.8 transcript indicate that TPA mediates concurrently positive and negative regulation of c-mos transcription. The results show that the c-mos gene is a TPA-modulated gene. In addition, a TPA-responsive element (GTGACTCA), which exists in the 5'-flanking region of c-mos gene of Balb/c mice [1,2], is suggested to be involved in this response. However, these changes were not accompanied by early marker changes associated with endodermal cell differentiation, i.e., morphological change, induction of plasminogen activator and suppression of glucose transport activity.