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Discrimination of Saccharides by a Simple Array.

N Maximilian Bojanowski1, Markus Bender1, Kai Seehafer1

  • 1Organisch-Chemisches Institut, Ruprecht-Karls-Universität Heidelberg, Im Neuenheimer Feld 270, 69120, Heidelberg, Germany.

Chemistry (Weinheim an Der Bergstrasse, Germany)
|April 13, 2017
PubMed
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We developed a simple fluorescence turn-on assay for detecting simple saccharides. This two-component probe system effectively discriminates and identifies nine different saccharides in aqueous solutions.

Area of Science:

  • Analytical Chemistry
  • Polymer Chemistry
  • Carbohydrate Chemistry

Background:

  • Fluorescence turn-on assays offer sensitive detection methods.
  • Conjugated polymers and boronic acids are useful in molecular recognition.
  • Previous work established quenching mechanisms between anionic polymers and cationic quenchers.

Purpose of the Study:

  • To develop a novel two-component probe system for saccharide detection.
  • To create a fluorescence turn-on assay for simple saccharides.
  • To demonstrate discrimination and identification of multiple saccharides using the developed system.

Main Methods:

  • Synthesized three boronic acid-functionalized benzyl viologens.
  • Prepared three conjugated polymers of the poly(aryleneethynylene) type.
Keywords:
carbohydratesconjugated polymersdiscriminationfluorescent probessensors

Related Experiment Videos

  • Formed nine non-fluorescent complexes by combining polymers and viologens.
  • Utilized a displacement assay in aqueous solutions for saccharide detection.

    • Main Results:

    • The developed probe system exhibits fluorescence turn-on upon interaction with saccharides.
    • Nine distinct simple saccharides were successfully discriminated.
    • The system demonstrated the ability to identify individual saccharides within the tested library.
    • The assay operates effectively in aqueous solutions.

    Conclusions:

    • A simple and effective two-component probe system for saccharide detection was successfully developed.
    • The system provides a sensitive fluorescence turn-on assay for simple saccharides.
    • This method allows for the discrimination and identification of multiple saccharides in aqueous media.