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Iodination of proteins on nitrocellulose blotting paper
M A Hernández1, R Padilla, J Avila
1Centro de Biología Molecular (CSIC-UAM), Universidad Autónoma, Madrid, Spain.
Journal of Biochemical and Biophysical Methods
|May 1, 1988
Abstract:
A procedure is described for radioiodination to a high specific activity of proteins immobilized on nitrocellulose membranes. After radioiodination, the proteins can be removed from the nitrocellulose to perform such structural analyses as proteolytic mapping. This method allows the detection of small amounts of protein recognized by immunoblotting and may be used to compare the structure of immunologically related proteins.