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High-yield production of pure tagatose from fructose by a three-step enzymatic cascade reaction
Seon-Hwa Lee1, Seung-Hye Hong1, Kyoung-Rok Kim1
1Department of Bioscience and Biotechnology, Konkuk University, Seoul, 05029, Republic of Korea.
Objective:
To produce tagatose from fructose with a high conversion rate and to establish a high-yield purification method of tagatose from the reaction mixture.
Results:
Fructose at 1 M (180 g l-1) was converted to 0.8 M (144 g l-1) tagatose by a three-step enzymatic cascade reaction, involving hexokinase, plus ATP, fructose-1,6-biphosphate aldolase, phytase, over 16 h with a productivity of 9 g l-1 h-1. No byproducts were detected. Tagatose was recrystallized from ethanol to a purity of 99.9% and a yield of 96.3%. Overall, tagatose at 99.9% purity was obtained from fructose with a yield of 77%.
Conclusion:
This is the first biotechnological production of tagatose from fructose and the first application of solvent recrystallization for the purification of rare sugars.