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Updated: Aug 11, 2026

A Colorimetric Method for Measuring Iron Content in Plants
Published on: September 7, 2018
Dispersive liquid-liquid microextraction coupled with digital image colorimetric analysis for detection of total iron
Bo Peng1, Guorong Chen1, Kai Li1
1Key Laboratory of Bioelectrochemistry & Environmental Analysis of Gansu Province, Key Laboratory of Eco-Environment-Related Polymer Materials, Ministry of Education, College of Chemistry and Chemical Engineering, Northwest Normal University, Lanzhou 730070, China.
Abstract:
A simple and low cost assay for total iron in various samples based on dispersive liquid-liquid microextraction (DLLME) coupled with digital scanning image analysis was proposed. Orthogonal experiment design was utilized to optimize the amount of extraction solvent and disperser solvent, O-phenanthroline concentration and buffer pH. Under the optimum conditions, the calibration curve was linear over the range of 0.047-1.0μgmL-1 (R2>0.99) of iron. The limit of detection (LOD) for iron was 14.1μgL-1 and limit of quantification (LOQ) was 46.5μgL-1. The relative standard deviations for seven replicate determinations of 0.5μgmL-1 of iron was 3.75%. The method was successfully applied for analysis of total iron in water and food samples without using any spectral instrument and it could have a potential industrial impact in developing fast and portable devices to analyze the iron content in water and certain foods.
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