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Related Experiment Videos

Temporal Changes in Gene Expression Profile during Mature Adipocyte Dedifferentiation.

Julie Anne Côté1,2,3, Frédéric Guénard3,4, Julie Lessard1

  • 1Centre de Recherche de l'Institut Universitaire de Cardiologie et de Pneumologie de Québec, 2725 Chemin Ste-Foy, Quebec City, QC, Canada G1V 4G5.

International Journal of Genomics
|April 15, 2017
PubMed
Summary

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Human mature adipocyte dedifferentiation in culture involves losing fat cell functions and gaining traits for cell growth. This study tracked gene expression changes during this process.

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Genomics

Background:

  • Mature adipocytes are crucial for energy homeostasis.
  • Understanding adipocyte dedifferentiation is key to regenerative medicine and metabolic research.
  • Ceiling culture provides a unique model for studying adipocyte behavior.

Purpose of the Study:

  • To investigate gene expression profiles during human mature adipocyte dedifferentiation.
  • To identify key molecular changes associated with the loss of adipocyte function.
  • To explore the transition towards a proliferative state in dedifferentiated adipocytes.

Main Methods:

  • Human subcutaneous (SC) and omental (OM) adipose tissue samples were used.
  • Adipocytes were cultured using the ceiling culture method to induce dedifferentiation.

Related Experiment Videos

  • Gene expression analysis was performed using Affymetrix arrays at multiple time points (days 0, 4, 7, 12).
  • Hierarchical clustering identified functional gene groups based on expression patterns.

    • Main Results:

    • Adipocyte function genes (e.g., LIPE, ADIPOQ, FABP4) showed decreased expression from day 7 to 12.
    • Genes related to cell proliferation and growth (e.g., WNT5A, FGF5) significantly increased from day 7 to 12.
    • Extracellular matrix remodeling genes (e.g., COL1A1, MMP1, TGFB1) exhibited differential expression throughout the dedifferentiation process.

    Conclusions:

    • Adipocyte dedifferentiation is characterized by a decline in mature adipocyte-specific gene expression.
    • The process involves an upregulation of genes associated with cell cycle progression and development.
    • Matrix remodeling plays a significant role during adipocyte dedifferentiation.