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Analysis of Plasmalogen Synthesis in Cultured Cells
Masanori Honsho1, Yukio Fujiki2
1Medical Institute of Bioregulation, Kyushu University, 3-1-1 Maidashi, Higashi-ku, Fukuoka, 812-8582, Japan.
Methods in Molecular Biology (Clifton, N.J.)
|April 15, 2017
Abstract:
Plasmalogen synthesis can be analyzed by metabolic labeling, followed by the separation of ethanolamine plasmalogens from glycerophospholipids on one-dimensional thin-layer chromatography. The vinyl-ether bond of plasmalogens is acid-labile, which allows separating plasmalogens as 2-acyl-glycerophospholipids from diacyl-glycerophospholipids.