Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Multiple forms of an angiogenesis factor: basic fibroblast growth factor.

D Moscatelli1, J Joseph-Silverstein, M Presta

  • 1Department of Cell Biology, New York University Medical Center, N.Y. 10016.

Biochimie
|January 1, 1988
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The nanostructured secretome.

Biomaterials science·2019
Same author

Polymer nanoparticles for the intravenous delivery of anticancer drugs: the checkpoints on the road from the synthesis to clinical translation.

Nanoscale·2018
Same author

Absence of LTBP-3 attenuates the aneurysmal phenotype but not spinal effects on the aorta in Marfan syndrome.

Biomechanics and modeling in mechanobiology·2018
Same author

All-Optical Reinforcement Learning In Solitonic X-Junctions.

Scientific reports·2018
Same author

Contribution of vascular endothelial growth factor receptor-2 sialylation to the process of angiogenesis.

Oncogene·2017
Same author

Comparison of 10 murine models reveals a distinct biomechanical phenotype in thoracic aortic aneurysms.

Journal of the Royal Society, Interface·2017
Same journal

Differential cardiac microRNA expression in anoxic Trachemys scripta elegans turtles.

Biochimie·2026
Same journal

Renal failure-driven luminal ammonia production impairs gut barrier function in CKD.

Biochimie·2026
Same journal

Conditional Knockout of Indoleamine 2, 3-Dioxygenase-1 in Osteoprogenitor Cells in Mice Results in Sex-dependent Differences in Bone Mass.

Biochimie·2026
Same journal

Sedentariness disrupts, while exercise restores, thermogenic and metabolic plasticity in inguinal adipose tissue of mice.

Biochimie·2026
Same journal

Chrononutrition as a modulator of retinal metabolic resilience: A translational framework linking circadian biology to ocular disease.

Biochimie·2026
Same journal

Heterologous expression, purification, and biophysical characterisation of the cobalt-dependent nitrile hydratase from Rhodococcus rhodochrous ATCC BAA-870.

Biochimie·2026
See all related articles

Researchers isolated a key angiogenesis factor, human basic fibroblast growth factor (bFGF), from placenta and hepatoma cells. This factor stimulates endothelial cell activity and promotes new blood vessel growth in vivo.

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Biochemistry

Background:

  • Angiogenesis, the formation of new blood vessels, is crucial for development and disease.
  • Identifying and characterizing angiogenesis factors is vital for therapeutic development.

Purpose of the Study:

  • To isolate and characterize an angiogenesis factor from human placenta and hepatoma cells.
  • To determine the identity and biological functions of the purified factor.

Main Methods:

  • Isolation and purification of angiogenesis factor from biological sources.
  • Assays for protease production, DNA synthesis, and endothelial cell motility.
  • In vivo angiogenesis assays.
  • Amino acid sequencing and molecular weight analysis.

Related Experiment Videos

Main Results:

  • An angiogenesis factor was isolated that stimulates protease production, DNA synthesis, and motility in capillary endothelial cells.
  • The purified factor was identified as human basic fibroblast growth factor (bFGF).
  • A higher molecular weight form of bFGF (Mr 25,000) was identified, which possesses similar biological activity and receptor affinity as the typical form (Mr 18,000) and can be converted to the smaller form.

Conclusions:

  • Human basic fibroblast growth factor (bFGF) is a potent angiogenesis factor.
  • Variations in bFGF N-termini may represent naturally processed forms or proteolytically generated species.
  • The Mr 25,000 bFGF may serve as a precursor to the Mr 18,000 form, indicating potential post-translational regulation.