Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Total Internal Reflection Fluorescence Microscopy01:05

Total Internal Reflection Fluorescence Microscopy

Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

<i>Morpho</i> butterfly-inspired optical diffraction, diffusion, and bio-chemical sensing.

RSC advances·2022
Same author

Multi-Analyte Detection Based on Integrated Internal and External Sensing Approach.

IEEE transactions on nanobioscience·2021
Same author

Mode-multiplex plasmonic sensor for multi-analyte detection.

Optics letters·2020
Same author

Precursors to non-invasive clinical dengue screening: Multivariate signature analysis of in-vivo diffuse skin reflectance spectroscopy on febrile patients in Malaysia.

PloS one·2020
Same author

Integrated multichannel Young's interferometer sensor based on long-range surface plasmon waveguides.

Optics express·2019
Same author

High Fluence Chromium and Tungsten Bowtie Nano-antennas.

Scientific reports·2019

Related Experiment Video

Updated: Jul 18, 2026

Surface Enhanced Raman Spectroscopy Detection of Biomolecules Using EBL Fabricated Nanostructured Substrates
11:44

Surface Enhanced Raman Spectroscopy Detection of Biomolecules Using EBL Fabricated Nanostructured Substrates

Published on: March 20, 2015

Diamond ring fiber for evanescent field exposure.

Wee Lit Ng, Wei Ru Wong, Ghafour Amouzad Mahdiraji

    Optics Letters
    |April 15, 2017
    PubMed
    Summary

    Diamond Ring Fibers (DRF) offer high evanescent field exposure for enhanced light-matter interactions. These fibers enable sensitive sensing applications with minimal signal loss.

    Area of Science:

    • Photonics
    • Optical Fiber Technology
    • Sensing Applications

    Background:

    • Evanescent field interactions are crucial for optical sensing.
    • Traditional optical fibers have limitations in maximizing evanescent field exposure.
    • Diamond Ring Fibers (DRF) present a novel structure for improved light-matter interaction.

    Purpose of the Study:

    • To propose and analyze Diamond Ring Fibers (DRF) for enhanced evanescent field exposure.
    • To investigate the trade-off between evanescent field exposure and confinement loss in DRFs.
    • To demonstrate the practical fabrication and characterization of DRFs.

    Main Methods:

    • Theoretical analysis of DRF waveguide parameters.
    • Fabrication of DRFs using the stack-and-draw fiber drawing technique.

    More Related Videos

    Multicolor Fluorescence Detection for Droplet Microfluidics Using Optical Fibers
    10:21

    Multicolor Fluorescence Detection for Droplet Microfluidics Using Optical Fibers

    Published on: May 5, 2016

    Evanescent Field Based Photoacoustics: Optical Property Evaluation at Surfaces
    10:21

    Evanescent Field Based Photoacoustics: Optical Property Evaluation at Surfaces

    Published on: July 26, 2016

    Related Experiment Videos

    Last Updated: Jul 18, 2026

    Surface Enhanced Raman Spectroscopy Detection of Biomolecules Using EBL Fabricated Nanostructured Substrates
    11:44

    Surface Enhanced Raman Spectroscopy Detection of Biomolecules Using EBL Fabricated Nanostructured Substrates

    Published on: March 20, 2015

    Multicolor Fluorescence Detection for Droplet Microfluidics Using Optical Fibers
    10:21

    Multicolor Fluorescence Detection for Droplet Microfluidics Using Optical Fibers

    Published on: May 5, 2016

    Evanescent Field Based Photoacoustics: Optical Property Evaluation at Surfaces
    10:21

    Evanescent Field Based Photoacoustics: Optical Property Evaluation at Surfaces

    Published on: July 26, 2016

  • Experimental mode analysis and simulation of confinement loss and evanescent field exposure.

    • Main Results:

    • DRFs achieve high evanescent field exposure up to 39.56%.
    • Negligible confinement loss was observed in the fabricated DRFs.
    • The study confirmed the suitability of DRFs for light-matter interaction applications.

    Conclusions:

    • Diamond Ring Fibers are a promising platform for advanced optical sensing.
    • DRFs offer a protected, long medium for efficient light-matter interactions.
    • The proposed fiber design facilitates the integration of sensing elements.