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Encapsulating Proteins in Nanoparticles: Batch by Batch or One by One.

Yi Liu1, Aoneng Cao1

  • 1Institute of Nanochemistry and Nanobiology, Shanghai University, Shanghai, China.

Methods in Enzymology
|April 17, 2017
PubMed
Summary
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Protein encapsulation in nanoparticles (NPs) enhances stability and expands applications. This chapter details methods for protein-loaded NPs, aiding researchers in selecting and adapting techniques for diverse biomedical and industrial uses.

Area of Science:

  • Biotechnology
  • Materials Science
  • Nanotechnology

Background:

  • Protein encapsulation in nanoparticles (NPs) enhances stability against denaturation and protease degradation.
  • This technique broadens the applications of natural proteins beyond their native biological contexts.
  • Nanoparticle-based protein delivery is crucial for biomedical and industrial advancements.

Purpose of the Study:

  • To provide detailed protocols for various protein-in-nanoparticle encapsulation methods.
  • To offer guidance on selecting and modifying these methods for specific research purposes.
  • To discuss the advantages, disadvantages, challenges, and future directions in protein-nanoparticle technology.

Main Methods:

  • Development of multiple methods for protein encapsulation within nanoparticles (NPs).
Keywords:
BiocatalysisBioimagingBionanotechnologyChitosanDrug deliveryEnzymeFluorescentImmobilizationNanoarmoringProtein encapsulationReverse microemulsionSilica

Related Experiment Videos

  • Methods include batch or single-particle encapsulation, covalent or noncovalent approaches.
  • Protocols cover NP sizes ranging from nanometers to over 100nm.

    • Main Results:

    • Detailed protocols with specific examples are provided for adopting and modifying encapsulation techniques.
    • Comparative analysis of advantages and disadvantages for each method is presented.
    • Guidelines for choosing appropriate methods based on application requirements are discussed.

    Conclusions:

    • Protein encapsulation in NPs offers significant improvements in protein stability and functionality.
    • The provided protocols and guidelines facilitate the broader adoption of protein-nanoparticle technologies.
    • Further research is needed to address current challenges and explore future applications in biocatalysis, bioimaging, and drug delivery.