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Rapid and efficient separation of glycoprotein using pH double-responsive imprinted magnetic microsphere
Jingfan Xie1, Guanqun Zhong1, Changqun Cai1
1Key Laboratory of Environmentally Friendly Chemistry and Applications of Ministry of Education, College of Chemistry, Xiangtan University, Xiangtan, Hunan 411105, China.
Talanta
|April 17, 2017
Summary
This study introduces pH double-responsive imprinted magnetic microspheres for rapid glycoprotein enrichment. This method offers efficient glycoprotein capture and release, aiding clinical diagnostics.
Area of Science:
- Biochemistry
- Materials Science
- Analytical Chemistry
Background:
- Glycoproteins are crucial biomarkers for disease diagnostics.
- Detecting low-abundance glycoproteins in complex samples without pretreatment remains challenging.
Purpose of the Study:
- To develop a rapid and efficient method for glycoprotein enrichment.
- To create pH double-responsive imprinted magnetic microspheres for glycoprotein separation.
Main Methods:
- Fabrication of imprinted polymer shells on magnetic microspheres using free radical polymerization.
- Incorporation of a pH-sensitive monomer (2-(Dimethylamino) ethyl methacrylate) and a boronate affinity monomer (4-vinylphenylbronic acid).
- Use of ovalbumin (OVA) as the template molecule for imprinting.
Main Results:
- The microspheres exhibited pH double-responsive behavior, enabling controlled capture and release of OVA.
- High absorption capacity (81.2 mg/g) was achieved in approximately 10 minutes.
- Demonstrated efficient enrichment of glycoproteins.
Conclusions:
- The developed method provides a novel approach for imprinting glycoproteins.
- The pH double-responsive imprinted magnetic microspheres show significant potential for glycoprotein detection in clinical diagnostics.