Related Experiment Video
Updated: Aug 12, 2026

Novel RNA-Binding Proteins Isolation by the RaPID Methodology
Published on: September 30, 2016
RNA-Binding Proteins Revisited - The Emerging Arabidopsis mRNA Interactome
Tino Köster1, Claudius Marondedze2, Katja Meyer1
1Molecular Cell Physiology, Faculty of Biology, Bielefeld University, 33615 Bielefeld, Germany.
Abstract:
RNA-protein interaction is an important checkpoint to tune gene expression at the RNA level. Global identification of proteins binding in vivo to mRNA has been possible through interactome capture - where proteins are fixed to target RNAs by UV crosslinking and purified through affinity capture of polyadenylated RNA. In Arabidopsis over 500 RNA-binding proteins (RBPs) enriched in UV-crosslinked samples have been identified. As in mammals and yeast, the mRNA interactomes came with a few surprises. For example, a plethora of the proteins caught on RNA had not previously been linked to RNA-mediated processes, for example proteins of intermediary metabolism. Thus, the studies provide unprecedented insights into the composition of the mRNA interactome, highlighting the complexity of RNA-mediated processes.
Related Concept Videos
Cooperative Binding of Transcription Regulators
Riboswitches
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
RNA Editing
Regulated mRNA Transport
Cooperative Binding of Transcription Regulators
Cell Signaling in Plants

