Transcriptomic analyses of human bronchial epithelial cells BEAS-2B exposed to atmospheric fine particulate matter...

Respiratory exposure is the major route of atmospheric PM_2.5 entering the human body. Epidemiological studies have indicated that exposure to PM_2.5 is associated with increased risk of pulmonary diseases, but the underlying mechanisms remain less clear. In this study, human bronchial epithelial cells (BEAS-2B) were used to investigate the toxic effect and gene expression changes induced by PM_2.5 collected from Beijing, China, based on microarray and following bioinformatic analyses. Gene ontology (GO) analysis indicated that PM_2.5 caused significant changes in gene expression patterns related to a series of important functions, covering gene transcription, signal transduction, cell proliferation, cellular metabolic processes, immune response, etc. Additionally, pathway analysis and signal-net analysis showed that PI3K/Akt, MAPK, and TNF signaling pathways were the most prominently significant pathways affected by PM_2.5, which play key roles in regulating cell proliferation, cell differentiation, cytoskeleton regulation, and inflammatory response. Finally, for the purpose of verifing the accuracy of microarray analysis, qRT-PCR was used to detect the expression of part key genes in the above signaling pathways, which were selected from the signal-net. Our study provided a large amount of information on the molecular mechanism that underling PM_2.5 caused pulmonary diseases, and follow-up researches are still needed for further exploration.