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Related Experiment Videos

Green listed-a CRISPR screen tool.

Sudeepta Kumar Panda1, Sanjay V Boddul1, Guillermina Yanek Jiménez-Andrade1

  • 1Department of Medicine, Solna, Karolinska Institutet, and Karolinska University Hospital, SE-171 76 Stockholm, Sweden.

Bioinformatics (Oxford, England)
|April 18, 2017
PubMed
Summary
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Green Listed is a web-based tool for designing custom CRISPR screens to study gene function. This tool enables rapid, targeted gene set selection for various research applications, including functional genomics and pathway analysis.

Area of Science:

  • Genomics
  • Molecular Biology
  • Bioinformatics

Background:

  • CRISPR/Cas9 gene editing is a powerful tool for functional genomics.
  • Designing custom CRISPR screens can be complex and time-consuming.

Purpose of the Study:

  • To develop a user-friendly web-based tool for designing custom CRISPR screens.
  • To facilitate the rapid selection of targeted gene sets for functional studies.

Main Methods:

  • Development of a web-based software tool named Green Listed.
  • Integration of gene set definition capabilities for custom CRISPR screen design.

Main Results:

  • Green Listed enables rapid design of custom CRISPR screens.
  • The tool supports targeting gene sets based on user-defined criteria (e.g., differential expression, pathway membership).

Related Experiment Videos

  • Facilitates the creation of targeted secondary screens.

    • Conclusions:

    • Green Listed streamlines the design of CRISPR-based functional genomics screens.
    • The tool enhances research efficiency by enabling rapid, custom gene set targeting.