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Related Experiment Videos

Inducing protein aggregation by extensional flow.

John Dobson1, Amit Kumar2,3, Leon F Willis2,3

  • 1School of Mechanical Engineering, University of Leeds, Leeds, LS2 9JT, United Kingdom.

Proceedings of the National Academy of Sciences of the United States of America
|April 19, 2017
PubMed
Summary

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This summary is machine-generated.

Hydrodynamic flow fields can cause protein unfolding and aggregation, even for stable proteins. This study reveals extensional flow readily exposes hidden protein sequences, driving aggregation based on protein type and flow conditions.

Area of Science:

  • Biophysics
  • Protein Chemistry
  • Chemical Engineering

Background:

  • Hydrodynamic flow fields' impact on protein stability is poorly understood.
  • Protein aggregation occurs in nature and during industrial manufacturing.
  • Relationships between flow, protein structure, and aggregation are unclear.

Purpose of the Study:

  • Assess extensional flow effects on protein aggregation.
  • Investigate aggregation of BSA, β2m, G-CSF, and mAbs.
  • Determine factors influencing flow-induced protein aggregation.

Main Methods:

  • Utilized a defined, quantified extensional flow field.
  • Exposed various proteins to flow for 0.36-1.8 ms.
  • Measured aggregation at low protein concentrations (0.5 mg mL-1).
Keywords:
aggregationantibodybioprocessingextensional flowunfolding

Related Experiment Videos

  • Demonstrated in situ labeling of buried cysteine in BSA under stress.

    • Main Results:

    • Extensional flow induced aggregation in multiple proteins.
    • Aggregation extent depended on strain rate, protein concentration, structure, and sequence.
    • Flow readily unfolded stable proteins, exposing aggregation-prone regions.
    • Aggregation occurred rapidly (0.36-1.8 ms) at low concentrations.

    Conclusions:

    • Extensional flow is a significant factor in protein aggregation.
    • Protein sequence and structure dictate susceptibility to flow-induced unfolding and aggregation.
    • Understanding these mechanisms is crucial for protein manufacturing and stability.