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Data for transcriptomic and proteomic analyses of leaves from Clematis terniflora DC. under binary stress
Bingxian Yang1,2, Qijie Guan1, Jingkui Tian1
1College of Biomedical Engineering & Instrument Science, Zhejiang University, Hangzhou 310027, China.
Data in Brief
|April 19, 2017
Summary
High UV-B irradiation and subsequent dark treatment boost secondary metabolites in Clematis terniflora. Transcriptomic and proteomic analyses reveal key gene and protein expression changes underlying this plant stress response.
Area of Science:
- Plant Science
- Molecular Biology
- Biochemistry
Background:
- UV-B radiation is a significant environmental stressor for plants.
- Secondary metabolites play crucial roles in plant defense and adaptation.
- Clematis terniflora DC. accumulates secondary metabolites under UV-B stress.
Purpose of the Study:
- To elucidate the molecular mechanisms behind secondary metabolite accumulation in Clematis terniflora DC. under UV-B stress.
- To investigate the combined effects of high UV-B irradiation and subsequent dark treatment on gene and protein expression.
- To identify key genes and proteins involved in the plant's stress response pathway.
Main Methods:
- Transcriptomic analysis using mRNA-sequencing.
- Proteomic analysis employing a gel-free/label-free technique.
- Quantitative reverse transcription polymerase chain reaction (qRT-PCR) for gene expression validation.
Main Results:
- Differential gene expression patterns were identified under UV-B irradiation and dark treatment.
- Significant changes in protein abundance were observed in response to the applied stresses.
- qRT-PCR confirmed the expression levels of selected genes.
Conclusions:
- Combined UV-B and dark treatment triggers a complex molecular response in Clematis terniflora.
- Transcriptomic and proteomic data provide insights into the regulation of secondary metabolite biosynthesis under stress.
- This study contributes to understanding plant adaptation strategies to environmental challenges.
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