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Updated: Jul 21, 2026

An Analytical Tool that Quantifies Cellular Morphology Changes from Three-dimensional Fluorescence Images

An Analytical Tool that Quantifies Cellular Morphology Changes from Three-dimensional Fluorescence Images

Published on: August 31, 2012

Multidimensional single-cell analysis based on fluorescence microscopy and automated image analysis.

Michael Sandmann¹, Martin Lippold², Franziska Saalfrank²

¹Institute for Food and Environmental Research (ILU e. V.), Arthur-Scheunert-Allee 40-41, 14558, Nuthetal, Germany. michael.sandmann@ilu-ev.de.

Analytical and Bioanalytical Chemistry

|April 19, 2017

Summary

A new single-cell analysis method using epifluorescence microscopy reveals significant cell-to-cell variations in chlorophyll content and density in green algae cultures. This heterogeneity impacts understanding algal physiology and biomass production.

Keywords:

Chlorophyll Multidimensional single-cell analysis Object recognition Wide-field fluorescence microscopy

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Analysis of Multidimensional Microscopy Data Using Cell-ACDC

Analysis of Multidimensional Microscopy Data Using Cell-ACDC

Published on: November 7, 2025

Area of Science:

Biotechnology
Cell Biology
Microscopy

Background:

Fast-growing microalgae are crucial for biomass production.
Understanding cellular heterogeneity is vital for optimizing algal cultivation and metabolic studies.

Purpose of the Study:

To develop and validate a single-cell analytical technology for evaluating fast-growing green algae.
To investigate cell-to-cell heterogeneity in key physiological parameters.

Main Methods:

Epifluorescence microscopy-based cytometry.
Automated image analysis with single-threshold discrimination.
Reference analyses for validation of object recognition, particle size, and chlorophyll determination.

Main Results:

The developed technique reliably assesses single-cell parameters.
Algal cells (Acutodesmus o.) exhibited significant heterogeneity in size, chlorophyll amount, and density.
High correlation between cell size and chlorophyll amount, but low correlation with chlorophyll density was observed.

Conclusions:

Single-cell analysis reveals substantial variations in the photosynthetic apparatus architecture and physiological state within algal populations.
This heterogeneity must be considered in systems biology models of algal biomass accumulation and metabolism.