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Dibutyryl cyclic AMP decreases expression of ED-A fibronectin by canine chondrocytes
N Burton-Wurster1, H R Leipold, G Lust
1James A. Baker Institute for Animal Health, New York State College of Veterinary Medicine, Cornell University, Ithaca 14853.
Abstract:
The presence of Extra Domain A (ED-A) in fibronectins from cartilage and from the culture medium of chondrocytes cultured under various conditions was determined with monoclonal antibody IST-9. Less than 2% of the fibronectin extracted from cartilage was fibronectin which contained the ED-A sequence (ED-A fibronectin). Chondrocytes placed in primary culture began to synthesize increased amounts of ED-A fibronectin (6% to 36% of the total fibronectin). Expression of ED-A fibronectin was selectively reduced when the chondrocytes were cultured in the presence of 0.5 mM dibutyryl cyclic AMP.